This is a time-lapse movie of an sp7:EGFP transgenic zebrafish opercle bone developing between 55 and 79 hpf (hours post-fertilization) imaged using spinning disk confocal microscopy. The embryo is orientated so that anterior is to the left, dorsal is to the top. The opercle initially forms as a cluster of a few cells. Cells are added posteriorly, so that the opercle elongates to form a "stick-like" structure.
This is a time-lapse movie of an sp7:EGFP transgenic zebrafish opercle bone developing between 72 and 100 hpf (hours post-fertilization) imaged using spinning disk confocal microscopy. The embryo is orientated so that anterior is to the left, dorsal is to the top. The opercle is initially a "stick-like" structure, but "fans-out" posteriorly.
This is a time-lapse movie of an sp7:EGFP transgenic zebrafish opercle bone developing between 60 and 72 hpf (hours post-fertilization) stained with the vital dye Alizarin Red, imaged using spinning disk confocal microscopy. The embryo is orientated so that anterior is to the left, dorsal is to the top. Movie A) The opercle initially forms as a cluster of a few cells. Movie A) Cells are added posteriorly, so that the opercle elongates to form a "stick-like" structure. Movie B) GFP-positive osteoblasts only C) Alizarin Red stain only. Matrix secretion extends posteriorly following the extension of GFP-positive cells.
Movie_1 Movie_2 Movie_3a Movie_3b Movie_3c