uo
Transgenic Mouse Facility tmf
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Rederivation, Cryopreservation, IVF

Service Price
UO
Investigators
Non-UO
Investigators
     
Rederivation on C57Bl/6J
$375
$450
Rederivation on ICR
$325
$400
Rederivation (females provided by PI)
$300
$375
Embryo Cryopreservation
$950
$1250
Sperm Cryopreservation
$300
$500
One time shot cryo
$600
$850
IVF/Rapid colony expansion
$1500
$2000
Resurrection of frozen embryos
$275
$350

 

Service Request

  1. Fill out a service request form and send to tmf@uoneuro.uoregon.edu.
  2. Please send a copy of your IACUC approved protocol and your IBC (Institutional Biosafety Committee) registration information.
  3. Rederivations: two proven breeder males age 2-8 month, four 3-4 week old females if provided by PI.
  4. Cryopreservation: five proven breeder males age 2-8 month, 15-20 3-4 week old females if provided by PI.
  5. IVF: two proven breeder males age 2-8 month, 15-20 3-4 week old females if provided by PI.

 

Rederivation Service Description
  1. Purchase egg donors and recipient females (week 1)
  2. Mate superovulated donors with stud males (week 2)
  3. Plug check for pseudopregnant foster mothers mated to vasectomized males
  4. Harvest eggs for transfer
  5. Incubate fertilized eggs O/N
  6. Ovarian transfer of 2-cell embryos (week 3)
  7. Wean, ear tag and take tail biopsy from resulting offspring (week 8)
  8. Transfer tail biopsies and mice to the client (week 9)

 

Embryo Cryopreservation Service Description
  1. Purchase females mice for egg donors (week 1)
  2. Mate superovulated donors with stud males (week 2)
  3. Harvest eggs for freezing
  4. Incubate fertilized eggs O/N (optional)
  5. Slow freeze 2-cell or 8-cell embryos in straws (week 3 and 4)
  6. Test thaw and transfer embryos into pseudopregnant foster mothers mated to vasectomized males (week 5)
  7. Wean, ear tag and take tail biopsy from resulting offspring (week 10)
  8. Transfer tail biopsies and transgenic founders to the client (week 11)

IVF Service Description

  1. Purchase egg donors females (week 1)
  2. Superovulate donors (week 2)
  3. Harvest eggs and sperm and use for IVF
  4. Incubate fertilized eggs O/N
  5. Transfer fertilized eggs into pseudopregnant foster mothers (week 3)
  6. Wean, ear tag and take tail biopsy from resulting offspring (week 8)
  7. Transfer tail biopsies and mice to the client (week 9)