Future Developments
These are few techniques that are planned to be developed at the TMF in order to reduce the time spent creating transgenic lines and their price. For a full screen copy of the slides below, double click on the title.
These are few techniques that are planned to be developed at the TMF in order to reduce the time spent creating transgenic lines and their price.
For a full screen copy of the slides below, double click on the title.
Lentiviral vector can provide a new faster way of making transgenic mice. Instead of pronuclear microinjection the virus carries the transgene.
The production of chimeras by aggregation can be a cheaper way of producing homologous recombinant mice.
Using tetraploid embryos, the rate of success of KO can be greatly increased:
The embryo derives exclusively from the ES cells which reduces the time and the chance of failure in transmission.
