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Two NB 4-1 clones in adjacent segments. The anterior clone is in T3 and posterior clone is in A1. The motoneuron is seen to extend by this stage in A1, but not in T3.
NB 4-1: Four ways to view the same clones upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns. upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone. lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background. lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP. In all cases, anterior is up.

Two NB 4-1 clones in adjacent segments.

The anterior clone is in T3 and posterior clone is in A1.

The motoneuron is seen to extend by this stage in A1, but not in T3.

NB 4-1:

Four ways to view the same clones

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

In all cases, anterior is up.