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The NB 3-3 clone is anterior and a 3-5 clone is in the next adjacent segment, posteriorly. The clones are at mid-stage 15. We chose these movies to demonstrate that the clones are remarkably similar at this stage, but can be distinguished by their relative positions in the hemisegment and by the fascicles to which they extend axons.
(NB 3-3 anteriorly and a NB 3-5 clone posteriorly.) Four ways to view the same clones: upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.Movie begins at dorsal surface of clone. upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone. lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background. lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP. In all cases, anterior is up.

The NB 3-3 clone is anterior and a 3-5 clone is in the next adjacent segment, posteriorly.

The clones are at mid-stage 15. We chose these movies to demonstrate that the clones are remarkably similar at this stage, but can be distinguished by their relative positions in the hemisegment and by the fascicles to which they extend axons.

(NB 3-3 anteriorly and a NB 3-5 clone posteriorly.)

Four ways to view the same clones:

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.Movie begins at dorsal surface of clone.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

In all cases, anterior is up.