This stage 17 NB 7-2 clone is in T2

Thoracic isoforms of the NB 7-2 clone form two discrete clusters of intersegmnetal interneurons. This is the only clone in which ipsilateral intersegmental interneurons form.

NB 7-2:

 

 

 

Four ways to view the same clones

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

 

In all cases, anterior is up.