This stage 17 NB 7-1 clone is in T1

The motoneuronal branch exiting SNd is visible to the right. NB 7-1 generates the largest of all clones, including as many as 40 cells. It generates 6-8 motoneurons and a large number of local interneurons as well as some glia.

The NB 7-1 clone is another example of a medially migrating cell that appears to act in pioneering the commissures for lineally related cells; in the case of NB 7-1, the medially migrating cell(s) is the well-described f(pCC).That cell is easily seen in these movies.

NB 7-4:

 

Four ways to view the same clones

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

 

In all cases, anterior is up.