The NB 4-1 clone is in T1; intersegmental interneurons are abbreviated anteriorly.

NB 4-1:

NB 4-1 generates large numbers of interneurons and a TN motoneuron in abdominal segments. In T1, there are more local interneurons in the anterior commissure and fewer intersegmental interneuronal projections.

Four ways to view the same clones

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns. Movie begins at dorsal surface of clone.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

 

In all cases, anterior is up.