The NB 4-2 clone is anterior to a NB 5-6 clone.

Both are in thoracic segments.

NB 5-6 at stage 17 generates an umbrella-like sheath over the lateral sides of the CNS.

NB 4-2 clone:

 

Four ways to view the same clones

 

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

 

In all cases, anterior is up.