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This is a stage 16 abdominal NB 6-1 clone. Primary interneuronal projections are established and the clone begins to look different from the NB 4-1, which it resembles strongly at stage 15.
Two ways to view the same clone. left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns. right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone. lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background. lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP. In all cases, anterior is up.

This is a stage 16 abdominal NB 6-1 clone.

Primary interneuronal projections are established and the clone begins to look different from the NB 4-1, which it resembles strongly at stage 15.

Two ways to view the same clone.

left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

In all cases, anterior is up.