These movies are of a thoracic NB 7-1 clone anteriorly and an MP2 clone posteriorly.

Thoracic clones frequently consist of 40 or even more cells. The mature SNd branch is seen to the right in these movies.

 

Abdominal NB 7-1 clone and an MP2 clone

 

Four ways to view the same clones

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

 

In all cases, anterior is up.