Abdominal NB 4-1 at late stage 16.

Putative Transverse Nerve Motoneuron is extending into the Transverse Nerve, but the bifurcation is not yet as robust as at stage 17.

NB 4-1

NB 4-1 generates the putative TN motoneuron; its bifurcating axon is apparent by stage 16. In other respects thoracic and abdominal segments seem identical.

Four ways to view the same clones.

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns. The movie begins at the dorsal surface of the clone.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

 

In all cases, anterior is up.