These two 7-1 clones are in upper abdominal segments.

Branches to SNd are clearly visible.

Both clones are at stage 17; except for the number of cells per clone, NB 7-1 does not generate segment -specific differences.

 

NB 7-1:

 

Four ways to view the same clones

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

 

In all cases, anterior is up.