Abdominal NB 3-5 clone.

These movies show the segmental nerve glial cell that we observe in this lineage.

NB 3-5

NB 3-5 generates a segmental nerve glial cell laterally.In abdominal segments there is only one cluster of interneurons; they project contralterally and appear to be primarily intersegmental.

Four ways to view the same clones

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

 

In all cases, anterior is up.