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Abdominal NB 4-1 clones generate the putative Transverse Nerve Motoneuron |
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NB 4-1 NB 4-1 generates a large number of intersegmental interneurons that project ipsilaterally and contralaterally, in both anterior and posterior directions. It also generates a large number of local interneurons. In abdominal segments, it generates one of the two horizontally bifurcating cells we find in the CNS. We believe this cell to be the Transverse Nerve motoneuron. (The other horizontally bifurcating cell is generated from NB 5-5 and is likely to be the Drosophila homolog of the Manduca Va cell, identified by O'Brien and Taghert in 1988.) Two ways to view the same clones upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.Movie begins at the dorsal surface of the clone. upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone. lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background. lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP. In both cases, anterior is up. |
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