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NB 2-1 forms during S4 and generates primarily local interneurons. Clones derived from NB 2-1 are the same in abdominal and thoracic segments.This clone has developed to stage 15.
Four ways to view the same clones upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns. upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone. lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background. lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP. In all cases, anterior is up.

NB 2-1 forms during S4 and generates primarily local interneurons.

Clones derived from NB 2-1 are the same in abdominal and thoracic segments.This clone has developed to stage 15.

Four ways to view the same clones

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

In all cases, anterior is up.