These movies are of an abdominal NB 1-2 clone at early stage 17.

 

 

Abdominal

NB 1-2 Clone:

There are three major axonal projections: 1) the anterior projection of the TB neuron in this segment's anterior commissure, the projection across the midline in this segment's posterior commissure and another projection across the midline in the next posterior segment's anterior commissure. The clone contains intersegmental interneurons and local interneurons, but no DC motoneurons.

 

Four ways to view the same clones

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

 

In all cases, anterior is up.