NB 5-5 generates what we believe to be the Drosophila homolog of the Va neurosecretory cell described by O'Brien and Taghert in Manduca.

This clone generates the second of the two horizontally bifurcating cells in the Drosophila CNS, the other being derived from NB 4-1. This cell has two bilaterally bifurcating processes which

 

NB 5-5

Four ways to view the same clones

 

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

 

In all cases, anterior is up.