NB 5-5 generates what we believe to be the Drosophila homolog of the Va neurosecretory cell described by O'Brien and Taghert in Manduca.

At stage 16, it is not yet extending to the Transverse Nerve.

Four ways to view the same clones

 

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

 

In all cases, anterior is up.