These movies are of a NB 6-1 clone in T2.

Note that ipsilateral interneuronal projections are not fully extended in this clone, but the contralateral intersegmental interneurons that project in the posterior direction do form in T2; comapre to the NB 6-1 clone of T1.

 

NB 6-1 clone in T2.

 

Four ways to view the same clones

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

 

In all cases, anterior is up.