NB 5-6 generates lateral subperineurial glia.

This clone is at stage 16; its interneuronal projections are not as complex as those seen in this lineage at stage 17.

The glia are almost fully differentiated by this stage.

NB 5-6 clone:

Four ways to view the same clones

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

 

In all cases, anterior is up.