MP3:

MP3 appears to generate interesgmental interneurons that are the homologs of the grasshopper H- cells. Their differentiation is similarly segment-specific: the long intersegmental interneuronal projections do not form in A6, for example.

Four ways to view the same clones

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

In all cases, anterior is up.