NB 5-2 generates a contralaterally-projecting motoneuron innervating muscle 12

and intersegmental and local interneurons.

It produces most of the axons of the posterior commissure.

This NB 5-2 clone is in the upper abdomen and is in an eagle-membrane-GFP background.

 

Four ways to view the same clones

 

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in egl-mGFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

 

In all cases, anterior is up. This clone is in the lower abdomen.