The NB 3-5 clone in T1 generates an Intersegmental Nerve Motoneuron that innervates muscles 3 and 9.

NB 3-5 clone

In T1 only, NB 3-5 generates a MN that innervates dorsal muscle targets. All thoracic NB 3-5 clones generate twice as many cells as abdominal NB 3-5 clones do, and these separate themselves into two discrete clusters, both ventral but one more lateral and anterior than the other.

 

There is a putative NB 3-3 clone immediately posterior to this NB 3-5 clone, but we did not identify the NB that generated it.

Four ways to view the same clones:

upper left panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

upper right panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

lower left panel is a confocal z-series of DiI-labeled clone in en-GFP background.

lower right panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

 

In all cases, anterior is up.