The NB 2-4 clone

In the center of the clone are two cells, always paired. The more medial of the pair is the only true bi-polar cell we observe in the insect CNS, previously never seen in insects. The more lateral cell in the pair is the contralaterally projecting MN that innervates muscle 8 in a unique way, possibly corresponding to the leukokinin positive cell described by Cantera and Nassl (1992).

There is a cluster of local interneurons laterally as well; these cross the midline in the anterior commissure with the MN and turn anteriorly only as far as the segment boundaries.

We occasionally observed a PNS sub-clone to be derived from NB 2-4, part of which is seen here, off to the right.

Four ways to view the same clones:

the top panel is a confocal z-series of DiI labeled clone; sections are 1.5 microns.

the second panel is a projection of z-series, rotated 45 degrees about y-axis. DiI alone.

the third panel is a confocal z-series of DiI-labeled clone in en-GFP background.

the bottom panel is a projection of the z-series, rotated 45 degrees about y-axis. DiI with en-GFP.

In all cases, anterior is up.